EVA1/MPZL2 Antibody (2E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00010205-M06

Novus Biologicals, part of Bio-Techne
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H00010205-M06
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EVA1 Antibodies »

Product Specifications

Immunogen

MPZL2 (NP_005788.1, 27 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VEIYTSRVLEAVNGTDARLKCTFSSFAPVGDALTVTWNFRPLDGGPEQFVFYYHIDPFQPMSGRFKDRVSWDGNPERYDASILLWKLQFDDNGTYTCQVKNPPDVDGVIG

Specificity

EVA1 - epithelial V-like antigen 1 (2E10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for EVA1/MPZL2 Antibody (2E10)

Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06]

Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06]

Immunocytochemistry/Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06] - Immunofluorescence of monoclonal antibody to EVA1/MPZL2 on HeLa cell. [antibody concentration 40 ug/ml]
Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06]

Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06]

Immunocytochemistry/Immunofluorescence: EVA1/MPZL2 Antibody (2E10) [H00010205-M06] - Analysis of monoclonal antibody to MPZL2 on HeLa cell. Antibody concentration 40 ug/ml
Sandwich ELISA: EVA1/MPZL2 Antibody (2E10) [H00010205-M06] - Detection limit for recombinant GST tagged MPZL2 is 1 ng/ml as a capture antibody.

Applications for EVA1/MPZL2 Antibody (2E10)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EVA1

Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Long Name

Epithelial V-Like Antigen 1

Alternate Names

MPZL2

Entrez Gene IDs

10205 (Human)

Gene Symbol

MPZL2

UniProt

NP_005788

Additional EVA1 Products

Product Documents for EVA1/MPZL2 Antibody (2E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EVA1/MPZL2 Antibody (2E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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