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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human Ecotropic Viral Integrations Site 5 using the numbering given in entry NP_005656.4 (GeneID 7813).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EVI5 Antibody

Immunoprecipitation: EVI5 Antibody [NB110-40620]

Immunoprecipitation: EVI5 Antibody [NB110-40620]

Immunoprecipitation: EVI5 Antibody [NB110-40620] - Detection of Human EVI5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EVI5 antibody NB110-40620 used for IP at 3 mcg/mg lysate. EVI5 was also immunoprecipitated by another rabbit anti-EVI5 antibody and NB110-40621, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for EVI5 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Optimal dilution to be determined by the researcher. Western Blot is not recommended. Use NB 110-40621 instead.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EVI5

EVI5 is a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics. It may act as an oncogene.

Alternate Names

dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5, ecotropic viral integration site 5 protein homolog, EVI-5, NB4SNeuroblastoma stage 4S gene protein

Entrez Gene IDs

7813 (Human)

Gene Symbol

EVI5

Additional EVI5 Products

Product Documents for EVI5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EVI5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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