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EVI5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82596

Novus Biologicals, part of Bio-Techne
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NBP1-82596
NBP1-82596-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MVTNKMTAAFRNPSGKQVATDKVAEKLSSTLSWVKNTVSHTVSQMASQVASPSTSLHTTSSSTTLSTPALSPSSPSQLSPD

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23910958). Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EVI5 Antibody

Immunocytochemistry/ Immunofluorescence: EVI5 Antibody [NBP1-82596]

Immunocytochemistry/ Immunofluorescence: EVI5 Antibody [NBP1-82596]

Immunocytochemistry/Immunofluorescence: EVI5 Antibody [NBP1-82596] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596]

Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596]

Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596]

Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596]

Immunohistochemistry-Paraffin: EVI5 Antibody [NBP1-82596] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in glial cells.

Applications for EVI5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EVI5

EVI5 is a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics. It may act as an oncogene.

Alternate Names

dJ846D11.1 (ecotropic viral integration site 5), ecotropic viral integration site 5, ecotropic viral integration site 5 protein homolog, EVI-5, NB4SNeuroblastoma stage 4S gene protein

Gene Symbol

EVI5

Additional EVI5 Products

Product Documents for EVI5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EVI5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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