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EVPL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37940

Novus Biologicals, part of Bio-Techne
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NBP2-37940
NBP2-37940-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EVPL Antibody

Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940]

Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940]

Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940] - Staining in human skin and skeletal muscle tissues using anti-EVPL antibody. Corresponding EVPL RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: EVPL Antibody [NBP2-37940]

Immunocytochemistry/ Immunofluorescence: EVPL Antibody [NBP2-37940]

Immunocytochemistry/Immunofluorescence: EVPL Antibody [NBP2-37940] - Staining of human cell line MCF7 shows localization to intermediate filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940]

Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940]

Immunohistochemistry-Paraffin: EVPL Antibody [NBP2-37940] - Staining of human skeletal muscle shows low expression as expected.

Applications for EVPL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EVPL

Alternate Names

210 KDa Cornified Envelope Precursor Protein, 210 KDa Paraneoplastic Pemphigus Antigen, envoplakin, EVPK, p210

Gene Symbol

EVPL

UniProt

Additional EVPL Products

Product Documents for EVPL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EVPL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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