Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 5H7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EWSR1 Antibodies »

Product Specifications

Immunogen

Full length recombinant human EWSR1 expressed in and purified from E. coli. [UniProt# Q01844]

Localization

Nucleus. Cytoplasm. Cell membrane. Note: Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for EWSR1 Antibody (5H7) [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This EWSR1 (5H7) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: EWSR1

EWSR1 (Ewing sarcoma breakpoint region 1) belongs to TET family (Tls, EWSR1, TafII) of RNA-binding proteins and it is implicated in transcriptional regulation for specific genes, mRNA splicing and transcription initiation. EWSR1 has the potential to associate with basal transcription factor TFIID and RNA polymerase II complex, and is required for proper localization of aurora B during mitosis as well as for maintaining mitotic spindle integrity. EWSR1 also associates with EP300/CREBBP as coactivator and EWSR1-EP300/CREBBP facilitates FOS activation as well as HNF4 genes activation. EWSR1 activates other transcription factors such as POU4F1 (or BRN3A, 13q13) and POU5F1 (or OCT4, 6p21) which regulate differentiation of neuronal cells. EWSR1 and CCNL1 are also interacting partners of TFIP11 which is functionally related to spliceosome and involved in pre-mRNA splicing. EWSR1 is required for cell survival in CNS and performs important functions in regulating genomic integrity and in RNA/microRNA's maturation processes. EWSR1 is ubiquity expressed with dominant localization in the nucleus and rarely in cytoplasm or various subcellular compartments, depending on the methylation state of its RNA-binding domain. Several gene families, mainly encoding for transcriptional regulators, can translocate with EWSR1 and the chimeric proteins have an aberrant activity interfering with different molecular pathways crucial for cell growth, differentiation and proliferation. EWSR1 is involved in several sarcoma translocations and soft tissue myoepithelial tumors.

Long Name

RNA-binding protein EWS

Alternate Names

EWS, EWS oncogene

Gene Symbol

EWSR1

Additional EWSR1 Products

Product Documents for EWSR1 Antibody (5H7) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EWSR1 Antibody (5H7) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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