EWSR1 Antibody

Immunoprecipitation: EWSR1 Antibody [NB200-182]

Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Simple Western: EWSR1 Antibody [NB200-182]

Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western: EWSR1 Antibody [NB200-182] -

The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: EWSR1 Antibody [NB200-182] -

The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: EWSR1 Antibody [NB200-182] -

Autophagic clearance of aberrantly accumulated cytoplasmic FUS restores protein homeostasis & ameliorates survival of SL P525L iPSC-derived neurons. a Confocal micrographs showing FUS-eGFP distribution before & after Torkinib treatment (above). Arrowhead indicates FUS-eGFP cytoplasmic accumulation in untreated neurites; arrow shows reduced FUS-eGFP cytoplasmic signal following torkinib treatment. Quantification of cytoplasmic FUS-eGFP signal intensity in acquired images (below) confirms clearance of mislocalized FUS-eGFP protein. Scale bar = 10 µm. b FRAP analysis performed on untreated versus torkinib-treated neurons shows comparable dynamics of FUS-eGFP recovery. n = 3. Error bars indicate SEM. CHX = cycloheximide. c WES capillary electrophoresis & d corresponding quantification of the indicated proteins in P525L SL neurons before & after torkinib treatment. Autophagy stimulation restores physiological levels. n = 4. Error bars indicate SEM. * & ** Correspond to p < 0.05 & 0.01, respectively. e 6 h of torkinib reduces apoptotic cell death identified by cleaved Caspase 3 staining. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.