Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EWSR1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 600 and the C-terminus (residue 656) of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EWSR1 Antibody

Western Blot: EWSR1 Antibody [NB200-183]

Western Blot: EWSR1 Antibody [NB200-183]

Western Blot: EWSR1 Antibody [NB200-183] - Detection of Human and Mouse EWS by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-183 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: EWSR1 Antibody [NB200-183]

Immunoprecipitation: EWSR1 Antibody [NB200-183]

Immunoprecipitation: EWSR1 Antibody [NB200-183] - Detection of human EWSR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWSR1 antibody NB200-183 used for IP at 6 ug per reaction. EWSR1 was also immunoprecipitated by rabbit anti-EWSR1 antibody NB200-182. For blotting immunoprecipitated EWSR1, NB200-183 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for EWSR1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EWSR1

This gene encodes a putative RNA binding protein of unknown function. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.

Long Name

RNA-binding protein EWS

Alternate Names

EWS, EWS oncogene

Entrez Gene IDs

2130 (Human)

Gene Symbol

EWSR1

UniProt

Q01844 (Human)

Additional EWSR1 Products

Product Documents for EWSR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EWSR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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