Exosome component 10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32870

Novus Biologicals, part of Bio-Techne
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NBP1-32870
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Avian - Chicken

Predicted:

Primate (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Exosome component 10 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Exosome component 10. The exact sequence is proprietary.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 24842995). Chimpanzee (99%).

Localization

Cytoplasm, Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Exosome component 10 Antibody

Western Blot: Exosome component 10 Antibody [NBP1-32870]

Western Blot: Exosome component 10 Antibody [NBP1-32870]

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EXOSC10 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870]

Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870]

Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870]

Western Blot: Exosome component 10 Antibody [NBP1-32870]

Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
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Applications for Exosome component 10 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-32870 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Exosome component 10

Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Could be an exonuclease . Plays a role in replication-dependent histone mRNA degradation. Mediates the association of MTR4, C1D and MPP6 wth the exosome and this complex is required for the maturation of 5.8S rRNA. Required for nucleolar localization of C1D

Alternate Names

autoantigen PM-SCL, EC 3.1.13.-, exosome component 10, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, p2, p3, p4, PM/Scl-100Autoantigen PM/Scl 2, PM-Scl, PMSCL2PMSCL, Polymyositis/scleroderma autoantigen 2, polymyositis/scleroderma autoantigen 2 (100kD), polymyositis/scleroderma autoantigen 2, 100kDa, RRP6, Rrp6p, RRP6Polymyositis/scleroderma autoantigen 100 kDa

Entrez Gene IDs

5394 (Human)

Gene Symbol

EXOSC10

UniProt

Q01780 (Human)

Additional Exosome component 10 Products

Product Documents for Exosome component 10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Exosome component 10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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