Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EYA2 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human eyes absent homolog 2 using the numbering given in entry NP_005235.3 (GeneID 2139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA2 Antibody

Western Blot: EYA2 Antibody [NBP1-46817]

Western Blot: EYA2 Antibody [NBP1-46817]

Western Blot: EYA2 Antibody [NBP1-46817] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody NBP1-46817 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody NBP1-46816, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46817]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46817]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46817] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for EYA2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 -1:2000

Immunohistochemistry-Paraffin

1:500 -1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EYA2

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Five transcript variants encoding three distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

EAB1EC 3.1.3.48, eyes absent (Drosophila) homolog 2, eyes absent homolog 2, eyes absent homolog 2 (Drosophila), MGC10614

Gene Symbol

EYA2

Additional EYA2 Products

Product Documents for EYA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EYA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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