EYA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46818

Novus Biologicals, part of Bio-Techne
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NBP1-46818
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all EYA3 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 30 of human eyes absent homolog 3 using the numbering given in entry NP_001981.2 (GeneID 2140).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA3 Antibody

Western Blot: EYA3 Antibody [NBP1-46818]

Western Blot: EYA3 Antibody [NBP1-46818]

Western Blot: EYA3 Antibody [NBP1-46818] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody NBP1-46818 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody NBP1-46819, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry: EYA3 Antibody [NBP1-46818]

Immunohistochemistry: EYA3 Antibody [NBP1-46818]

Immunohistochemistry: EYA3 Antibody [NBP1-46818] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818]

Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818]

Immunohistochemistry-Paraffin: EYA3 Antibody [NBP1-46818] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.

Applications for EYA3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EYA3

The EYA3 gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. A similar protein in mice acts as a transcriptional activator. [provided by RefSeq]

Alternate Names

DKFZp686C132, EC 3.1.3.48, eyes absent (Drosophila) homolog 3, eyes absent 3, eyes absent homolog 3, eyes absent homolog 3 (Drosophila)

Gene Symbol

EYA3

Additional EYA3 Products

Product Documents for EYA3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EYA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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