EYA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85547

Novus Biologicals, part of Bio-Techne
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NBP1-85547
NBP1-85547-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EYA4 Antibodies »

Product Summary for EYA4 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TGGENMTVLNTADWLLSCNTPSSATMSLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA4 Antibody

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547] - analysis in human cell lines HeLa and MCF-7 using Anti-EYA4 antibody. Corresponding EYA4 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547]

Western Blot: EYA4 Antibody [NBP1-85547] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-85547] - Staining of human heart muscle shows distinct cytoplasmic positivity in myocytes.

Applications for EYA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EYA4

EYA4 is thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. It may be involved in development of the eye.

Alternate Names

CMD1J, DFNA10, dJ78N10.1 (eyes absent), EC 3.1.3.48, eyes absent (Drosophila) homolog 4, eyes absent homolog 4, eyes absent homolog 4 (Drosophila)

Gene Symbol

EYA4

Additional EYA4 Products

Product Documents for EYA4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EYA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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