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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2A Clone # 612834

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2

Asn298-Tyr434
Accession # Q8NCQ5-2

Specificity

Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for F-box protein 15/FBXO15 Antibody (612834) [CoraFluor™ 1]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: F-box protein 15/FBXO15

FBXO15 (F-Box Protein 15) belongs to the F-box protein family which are involved in protein ubiquitination. FBXO15 is regulated by SOX2-OCT4 and POU5F1. FBXO15 is known to have interactions with SKP1, CUL1, BTRC, CCNF and FBXL15.

Alternate Names

Fbox protein 15, FBX15, FBXO15

Gene Symbol

FBXO15

Additional F-box protein 15/FBXO15 Products

Product Documents for F-box protein 15/FBXO15 Antibody (612834) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for F-box protein 15/FBXO15 Antibody (612834) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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