F4/80 Antibody (PSH01-87)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32324
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-87
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within mouse F4/80 aa 1-650 / 931. (Uniprot: Q61549)
Localization
Cell membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for F4/80 Antibody (PSH01-87)
Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324]
Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/1,000 dilution.Lane 1: RAW264.7(no heat) cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 102 kDa
Observed band size: 160 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324]
Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32324, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324]
Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/5,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32324) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for F4/80 Antibody (PSH01-87)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: F4/80
Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).
References
1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7
2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15
Alternate Names
DD7A5-7, Gpf480, Ly71
Gene Symbol
Adgre1
Additional F4/80 Products
Product Documents for F4/80 Antibody (PSH01-87)
Product Specific Notices for F4/80 Antibody (PSH01-87)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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