Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Bacteria - Escherichia coli, Nematode - Caenorhabditis elegans

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot

Cited:

ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # Cl:A3-1

Concentration

1.0 mg/ml

View all F4/80 Antibodies »

Product Specifications

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice

Localization

Cell surface

Marker

Macrophage Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for F4/80 Antibody (Cl:A3-1)

Immunohistochemistry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Immunohistochemistry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence or absence of Leptospira infection using various leukocyte markers. We determined the number of F4/80+ T cells per millimeter squared of kidney. Data are mean SEM of 4-5 mice per group. Scale bar represents 400x. Statistics by two-tailed paired t-test *p < 0.05, *** p < 0.001. Data is representative of one of two experiments. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pntd.0005870) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Immunocytochemistry/ Immunofluorescence: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.
Flow Cytometry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Flow Cytometry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404]

Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
View 9 more images

Applications for F4/80 Antibody (Cl:A3-1)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500RadioImmunoassay 1:100-1:2000

Radioimmunoassay

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
This product requires pretreatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. E. coli reactivity reported in scientific literature (PMID:31181736).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NB600-404 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: F4/80

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Alternate Names

DD7A5-7, Gpf480, Ly71

Entrez Gene IDs

13733 (Mouse)

Gene Symbol

ADGRE1

UniProt

Q61549

Additional F4/80 Products

Product Documents for F4/80 Antibody (Cl:A3-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for F4/80 Antibody (Cl:A3-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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