FABP2/I-FABP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59746

Novus Biologicals, part of Bio-Techne
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NB100-59746
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Fish

Cited:

Mouse, Fish

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all FABP2/I-FABP Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EGVEAKRIFKKD corresponding to C-Terminus according to NP_000125.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22790967). Fish reactivity reported in multiple pieces of scientific literature. Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FABP2/I-FABP Antibody

Western Blot: FABP2/I-FABP Antibody [NB100-59746]

Western Blot: FABP2/I-FABP Antibody [NB100-59746]

Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum (A) and (0.003ug/ml) Ileum (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746]

Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746]

Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for FABP2/I-FABP Antibody

Application
Recommended Usage

Flow Cytometry

10ug/ml

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.001- 0.003 ug/ml
Application Notes
Western blot: Approx 15-16kDa band observed in Human Ileum and Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). Recommended concentration: 0.001-0.003ug/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FABP2/I-FABP

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Entrez Gene IDs

2169 (Human); 14079 (Mouse)

Gene Symbol

FABP2

UniProt

P12104

Additional FABP2/I-FABP Products

Product Documents for FABP2/I-FABP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FABP2/I-FABP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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