FABP4/A-FABP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33616

Novus Biologicals, part of Bio-Techne
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NBP1-33616
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FABP4/A-FABP Antibodies »

Product Summary for FABP4/A-FABP Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FABP4/A-FABP. The exact sequence is proprietary.

Localization

Cytoplasm, Nucleus

Marker

Adipocyte Differentiation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FABP4/A-FABP Antibody

Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616]

Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616]

Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody [NBP1-33616] - Analysis of paraformaldehyde-fixed HeLa, using FABP4 (NBP1-33616) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-33616]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-33616]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-33616] - FABP4 antibody detects FABP4 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. FABP4 stained by FABP4 antibody diluted at 1:500.
Western Blot: FABP4/A-FABP Antibody [NBP1-33616]

Western Blot: FABP4/A-FABP Antibody [NBP1-33616]

Western Blot: FABP4/A-FABP Antibody [NBP1-33616] - Unstimulated and stimulated 3T3-L1 whole cell extracts (20 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FABP4 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.
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Applications for FABP4/A-FABP Antibody

Application
Recommended Usage

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FABP4/A-FABP

Fatty acid binding protein 4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

Long Name

Fatty Acid-Binding Protein 4

Alternate Names

A-FABP, AFABP, aP2

Gene Symbol

FABP4

Additional FABP4/A-FABP Products

Product Documents for FABP4/A-FABP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FABP4/A-FABP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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