FACA/FANCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2564

Novus Biologicals, part of Bio-Techne
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NB100-2564
NB100-2564SS
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all FACA/FANCA Antibodies »

Product Specifications

Immunogen

A synthetic peptide within residues 1200-1300 of the human FANCA protein. [Swiss-Prot# O15360]

Localization

The major form is nuclear. The minor form is cytoplasmic.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FACA/FANCA Antibody

Western Blot: FACA/FANCA Antibody [NB100-2564]

Western Blot: FACA/FANCA Antibody [NB100-2564]

Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.

Applications for FACA/FANCA Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This FANCA antibody is useful for Western Blot, where a band is seen at approximately 170 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FACA/FANCA

FANCA (Fanconi anemia group A protein) is a DNA repair protein which plays a key role in post-replication repair, cell cycle checkpoint function, interstrand DNA cross-link repair and maintenance of chromosome stability. FANCA belongs to multisubunit FA complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM) which is absent in FA patients. When complexed with FANCF, FANCG and FANCL (but not with FANCC, nor FANCE), FANCA interacts with HES1 and this interaction is crucial for the stability as well as nuclear localization of FA core complex proteins. FANCA-FANCC/FANCG complex may include EIF2AK2 and HSP70 also and it directly interacts with FAAP20/C1orf86. Localized mainly in the nucleus and cytoplasm, it gets phosphorylated upon DNA damage through ATM/ATR. FANCA is a most frequently mutated gene in FA (representing 60-70% of the cases) and over 350 unique mutations have been reported which includes large genomic deletions mediated by unusually high density of ALU repetitions found in its genomic sequence. FANCA sequence alterations or its altered expression has been associated with ovarian cancer and leukemia, and homozygous mutations of FANCA have been linked to esophageal cancer. Defects in FANCA are a cause of Fanconi anemia (FA), a rare disorder characterized by physical abnormalities, bone marrow failure (BMF), increased risk of malignancies, and cellular hypersensitivity to DNA cross-linking agents.

Long Name

Fanconi Anemia, Complementation Group A

Alternate Names

FAA, FAH, FANCA, FANCH

Entrez Gene IDs

2175 (Human)

Gene Symbol

FANCA

UniProt

O15360 (Human)

Additional FACA/FANCA Products

Product Documents for FACA/FANCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FACA/FANCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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