Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # F8C 27.4 (2A5)

Concentration

1.0 mg/ml

View all Factor VIII Antibodies »

Product Specifications

Immunogen

Purified human factor VIII

Reactivity Notes

Human

Specificity

NB100-62559 recognizes the 83 kDa light chain of Factor VIII. It does not cross-react with Von Willebrand factor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Factor VIII Antibody (F8C 27.4 (2A5))

Application
Recommended Usage

ELISA

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Factor VIII

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Alternate Names

AHF, Antihemophilic factor, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, DXS1253E, F8Ccoagulation factor VIII, factor VIII F8B, FVIII, HEMAF8B, Procoagulant component

Entrez Gene IDs

2157 (Human)

Gene Symbol

F8

UniProt

P00451 (Human)

Additional Factor VIII Products

Product Documents for Factor VIII Antibody (F8C 27.4 (2A5))

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Factor VIII Antibody (F8C 27.4 (2A5))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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