Factor VIII Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-91761

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Factor VIII Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of human Factor VIII (between amino acids 2100-2250) [UniProt P00451]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

267 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Factor VIII Antibody - BSA Free

Western Blot: Factor VIII AntibodyBSA Free [NB100-91761]

Western Blot: Factor VIII AntibodyBSA Free [NB100-91761]

Western Blot: Factor VIII Antibody [NB100-91761] - Detection of Factor VIII protein in extracts from human umbilical vein endothelial cells (HUVECs).
Immunocytochemistry/ Immunofluorescence: Factor VIII Antibody - BSA Free [NB100-91761]

Immunocytochemistry/ Immunofluorescence: Factor VIII Antibody - BSA Free [NB100-91761]

Immunocytochemistry/Immunofluorescence: Factor VIII Antibody [NB100-91761] - Factor VIII antibody was tested 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.
Immunohistochemistry-Paraffin: Factor VIII Antibody - BSA Free [NB100-91761]

Immunohistochemistry-Paraffin: Factor VIII Antibody - BSA Free [NB100-91761]

Immunohistochemistry-Paraffin: Factor VIII Antibody [NB100-91761] - Staining of Factor VIII in a formalin fixed and paraffin embedded tissue section of mouse bladder. The primary antibody was used at 1:100 dilution and the detection was performed using DAB with hematoxylin counterstaining.
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Applications for Factor VIII Antibody - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Factor VIII

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin, and Factors X, IX, V, and VIII), which are involved in a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation factor VIII is a 2351 amino acid, non-covalent heterodimer, that circulates as an inactive procofactor. When catalyzed by thrombin, factor VIII is converted to active Factor VIIIa, which associates with factor IXa. This complex catalyzes the conversion of factor X to factor Xa. This results in the amplification phase of coagulation.

Alternate Names

AHF, Antihemophilic factor, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, DXS1253E, F8Ccoagulation factor VIII, factor VIII F8B, FVIII, HEMAF8B, Procoagulant component

Entrez Gene IDs

2157 (Human); 14069 (Mouse)

Gene Symbol

F8

UniProt

P00451 (Human)

Additional Factor VIII Products

Product Documents for Factor VIII Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Factor VIII Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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