Factor XII Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-94203
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Summary for Factor XII Antibody
Immunogen
A synthetic peptide made to an internal portion of the human F12 protein (between residues 50-150) [UniProt P00748]
Localization
Secreted. Staining is also seen in the nucleus, not the nucleoli.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Factor XII Antibody
![Western Blot: Factor XII Antibody [NBP1-94203]](https://resources.bio-techne.com/images/products/F12-Antibody-Western-Blot-NBP1-94203-img0002.jpg "Western Blot: Factor XII Antibody [NBP1-94203]")
Western Blot: Factor XII Antibody [NBP1-94203]
Western Blot: Factor XII/F12 Antibody [NBP1-94203] - WB analysis of F12 in 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.![Immunocytochemistry/ Immunofluorescence: Factor XII Antibody [NBP1-94203]](https://resources.bio-techne.com/images/products/Factor-XII-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-94203-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Factor XII Antibody [NBP1-94203]")
Immunocytochemistry/ Immunofluorescence: Factor XII Antibody [NBP1-94203]
Immunocytochemistry/Immunofluorescence: Factor XII Antibody [NBP1-94203] - F12 antibody was tested in NIH/3T3 cells with FITC (green). Nuclei were counterstained with DAPI (blue).![Immunohistochemistry: Factor XII Antibody [NBP1-94203]](https://resources.bio-techne.com/images/products/Factor-XII-Antibody-Immunohistochemistry-NBP1-94203-img0005.jpg "Immunohistochemistry: Factor XII Antibody [NBP1-94203]")
Immunohistochemistry: Factor XII Antibody [NBP1-94203]
Immunohistochemistry: Factor XII Antibody [NBP1-94203] - IHC analysis of F12 in human renal cancer using DAB with hematoxylin counterstain.Applications for Factor XII Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:400
Western Blot
1:1000
Application Notes
This Factor XII/F12 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, bands are seen ~67, 50, and 140kDa representing the full product, a clevage chain product, and a possible dimer. In ICC/IF, cytoplasmic staining was observed in NIH/3T3 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of human renal cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Factor XII
Alternate Names
beta-factor XIIa part 1, coagulation factor XII, coagulation factor XII (Hageman factor), coagulation factor XIIa heavy chain, coagulation factor XIIa light chain, EC 3.4.21, EC 3.4.21.38, F12, HAE3, HAEX, HAF, HAF beta-factor XIIa part 2, Hageman factor
Entrez Gene IDs
2161 (Human)
Gene Symbol
F12
UniProt
Additional Factor XII Products
Product Documents for Factor XII Antibody
Product Specific Notices for Factor XII Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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