FADD Antibody (10L6M0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15951

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15951-100ul
NBP3-15951-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10L6M0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FADD Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of mouse FADD (NP_034305.1). MDPFLVLLHSLSGSLSGNDLMELKFLCRERVSKRKLERVQSGLDLFTVLLEQNDLERGHTGLLRELLASLRRHDLLQRLDDFEAGTATAAPPGEADLQVAFDIVCDNVGRDWKRLARELKVSEAKMDGIEEKYPRSLSERVRESLKVWKNAEKKNASVAGLVKALRTCRLNLVADLVEEAQESVSKSENMSPVLRDSTVSSSETP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FADD Antibody (10L6M0)

Western Blot: FADD Antibody (10L6M0) [NBP3-15951]

Western Blot: FADD Antibody (10L6M0) [NBP3-15951]

Western Blot: FADD Antibody (3X9R6) [NBP3-15951] - Western blot analysis of extracts of various cell lines, using FADD Rabbit mAb (NBP3-15951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: FADD Antibody (10L6M0) [NBP3-15951]

Immunohistochemistry-Paraffin: FADD Antibody (10L6M0) [NBP3-15951]

Immunohistochemistry-Paraffin: FADD Antibody (3X9R6) [NBP3-15951] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FADD Rabbit mAb (NBP3-15951) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: FADD Antibody (10L6M0) [NBP3-15951]

Immunohistochemistry-Paraffin: FADD Antibody (10L6M0) [NBP3-15951]

Immunohistochemistry-Paraffin: FADD Antibody (3X9R6) [NBP3-15951] - Immunohistochemistry of paraffin-embedded rat spleen using FADD Rabbit mAb (NBP3-15951) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for FADD Antibody (10L6M0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FADD

Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.

Long Name

Fas-associated Death Domain Protein

Alternate Names

MORT1

Gene Symbol

FADD

Additional FADD Products

Product Documents for FADD Antibody (10L6M0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FADD Antibody (10L6M0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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