Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FAF1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAF1. The immunogen is located within the last 50 amino acids of FAF1. Amino Acid Squence: STFPRRDVTQLDPNKSLLE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FAF1 Antibody - BSA Free

Western Blot: FAF1 AntibodyBSA Free [NBP1-76745]

Western Blot: FAF1 AntibodyBSA Free [NBP1-76745]

Western Blot: FAF1 Antibody [NBP1-76745] - Analysis of FAF1 in THP-1 cell lysate with FAF1 antibody at (A) 1 and (B) 2 ug/mL.
FAF1 Antibody - BSA Free

Immunohistochemistry: FAF1 Antibody - BSA Free [NBP1-76745] -

Immunohistochemistry: FAF1 Antibody - BSA Free [NBP1-76745] - Immunohistochemistry of FAF1 in rat spleen tissue with FAF1 antibody at 2.5 u/mL.
FAF1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FAF1 Antibody - BSA Free [NBP1-76745] -

Immunocytochemistry/ Immunofluorescence: FAF1 Antibody - BSA Free [NBP1-76745] - Immunofluorescence of FAF1 in rat spleen tissue with FAF1 antibody at 2 ug/mL.

Applications for FAF1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAF1

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-kappaB activity in transfected 293 cells, inhibits NF-kappaB activity induced by TNF-alpha, IL-1beta and lipopolysaccharide, and prevents NF-kappaB translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-kappaB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.

Alternate Names

CGI-03, Fas (TNFRSF6) associated factor 1, HFAF1s, TNFRSF6-associated factor 1, UBX domain protein 3A, UBX domain-containing protein 12, UBX domain-containing protein 3A, UBXD12FLJ37524, UBXN3AhFAF1FAS-associated factor 1

Gene Symbol

FAF1

UniProt

NP_008982 (Human)

Additional FAF1 Products

Product Documents for FAF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FAF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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