FAK Antibody (10H7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22168

Novus Biologicals, part of Bio-Techne
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NBP2-22168
NBP2-22168-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10H7

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all FAK Antibodies »

Product Summary for FAK Antibody (10H7) - BSA Free

Immunogen

Purified recombinant fragment of human FAK expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Focal Adhesion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FAK Antibody (10H7) - BSA Free

Western Blot: FAK Antibody (10H7)BSA Free [NBP2-22168]

Western Blot: FAK Antibody (10H7)BSA Free [NBP2-22168]

Western Blot: FAK Antibody (10H7) [NBP2-22168] - Western blot analysis using FAK mouse mAb against A549 (1) and NIH/3T3 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: FAK Antibody (10H7) - BSA Free [NBP2-22168]

Immunocytochemistry/ Immunofluorescence: FAK Antibody (10H7) - BSA Free [NBP2-22168]

Immunocytochemistry/Immunofluorescence: FAK Antibody (10H7) [NBP2-22168] - Immunofluorescence analysis of B16 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: FAK Antibody (10H7) - BSA Free [NBP2-22168]

Immunohistochemistry-Paraffin: FAK Antibody (10H7) - BSA Free [NBP2-22168]

Immunohistochemistry-Paraffin: FAK Antibody (10H7) [NBP2-22168] - Immunohistochemical analysis of paraffin-embedded cervices tumour using FAK mouse mAb with DAB staining

Applications for FAK Antibody (10H7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAK

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a "Focal Adhesion Targeting" (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Gene Symbol

PTK2

Additional FAK Products

Product Documents for FAK Antibody (10H7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FAK Antibody (10H7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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