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FAK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84750

Novus Biologicals, part of Bio-Techne
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NBP1-84750
NBP1-84750-25ul

Key Product Details

Species Reactivity

Validated:

Human, Porcine

Cited:

Porcine

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRE

Reactivity Notes

Porcine reactivity reported in the scientific literature (PMID: 30257480).

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAK Antibody

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression and metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta1 (a) or beta4 (b) receptor (green) and FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/19/10/2912), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining shown in green.

Applications for FAK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAK

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Gene Symbol

PTK2

Additional FAK Products

Product Documents for FAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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