FAK [p Tyr576] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13071
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0003.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary.
Modification
p Tyr 576
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for FAK [p Tyr576] Antibody
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0008.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]
Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.![Immunocytochemistry/ Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13071-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071]")
Immunocytochemistry/ Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071]
Immunocytochemistry/Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0001.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]
Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:3000.![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0004.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0005.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0006.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]](https://resources.bio-techne.com/images/products/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0007.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071]")
![FAK [p Tyr576] Antibody](https://resources.bio-techne.com/images/products/nbp3-13071_rabbit-fak-p-tyr-576-pab-91020242041236.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] -")
![FAK [p Tyr576] Antibody](https://resources.bio-techne.com/images/products/nbp3-13071_rabbit-fak-p-tyr-576-pab-9102024205680.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] -")
![FAK [p Tyr576] Antibody](https://resources.bio-techne.com/images/products/nbp3-13071_rabbit-fak-p-tyr-576-pab-910202420115066.jpg "Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] -")
Applications for FAK [p Tyr576] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAK
Long Name
Focal adhesion kinase 1
Alternate Names
FADK1, PTK2
Gene Symbol
PTK2
Additional FAK Products
Product Specific Notices for FAK [p Tyr576] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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