FAM134B Antibody

Immunocytochemistry/ Immunofluorescence: FAM134B Antibody [NBP2-55248]

Immunocytochemistry/Immunofluorescence: FAM134B Antibody [NBP2-55248] - Staining of human cell line HeLa shows localization to nuclear bodies & endoplasmic reticulum. Antibody staining is shown in green.

Western Blot: FAM134B Antibody [NBP2-55248] -

Western Blot: FAM134B Antibody [NBP2-55248] - Palmitate inhibits EBSS-induced ER-phagy. a–c Cells transiently expressing mRFP-LC3 were starved in EBSS for 3 h in the presence or absence of palmitate (0.1 mM) & were co-treated for 3 h with Baf A1. Treated cells were stained for the ER marker KDEL. Representative micrographs (a) & quantification (b) of colocalization between mRFP-LC3 & KDEL (n = 10 per each group). Scale bar, 10 μm. Data are mean ± SEM; ****p < 0.0001 vs. control, ###p = 0.0002, ####p < 0.0001, ††††p < 0.0001. c The ratio of the average number of KDEL-positive mRFP-LC3 puncta to the total number of mRFP-LC3 puncta per cell (n = 10 per each group). Data are mean ± SEM; ****p < 0.0001 vs. control, ####p < 0.0001, ††††p < 0.0001. d & e Immunoblotting analysis (d) & quantification (e) of ER-phagy receptors & LC3-II in cells starved in EBSS for 3 h in the presence or absence of palmitate (0.1 mM) & were co-treated for 3 h with Baf A1 (FAM134B; n = 8 for control, PA, EBSS, PA + EBSS, n = 5 for control + Baf A1, PA + Baf A1, EBSS + Baf A1, PA + EBSS + Baf A1, RTN3; n = 4 per each group, CCPG1; n = 3 per each group, LC3-II; n = 9 for control, PA, EBSS, PA + EBSS, n = 4 for control + Baf A1, PA + Baf A1, EBSS + Baf A1, PA + EBSS + Baf A1). Data are mean ± SEM; FAM134B; ****p < 0.0001 vs. control, ##p = 0.0016, ††p = 0.0094, CCPG1; ****p < 0.0001 vs. control, LC3-II; *p = 0.0331, ****p < 0.0001 vs. control, ###p = 0.0002 at PA vs. PA + Baf A1, ###p = 0.0005 at PA + EBSS vs. PA + EBSS + Baf A1, ####p < 0.0001, †p = 0.0203. n.s., no significant difference Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33823883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: FAM134B Antibody [NBP2-55248] -

Western Blot: FAM134B Antibody [NBP2-55248] - Palmitate impairs ER-phagy. a & b Immunoblotting analysis (a) & quantification (b) of ER-phagy receptors (FAM134B, RTN3, & CCPG1) in cells treated with 0.1 mM palmitate for the indicated times (FAM134B & RTN3; n = 4 per each group, CCPG1; n = 3 per each group). Data are mean ± SEM; FAM134B; **p = 0.0053, ***p = 0.0005 vs. control, CCPG1; *p = 0.0388, ***p = 0.0004 vs. control. c & d Cells transiently expressing mRFP-LC3 were treated with 0.1 mM palmitate for the indicated times in the presence or absence of Baf A1 (200 nM). Treated cells were stained for the ER marker KDEL. Representative micrographs (c) & quantification (d) of colocalization between mRFP-LC3 & KDEL (n = 13 per each group). Scale bar, 10 μm. Data are mean ± SEM; **p = 0.0029, ***p = 0.0008 vs. control, ##p = 0.0025. n.s., no significant difference Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33823883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.