FAM219B Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26092
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FAM219B Antibody - Azide and BSA Free
Immunogen
Produced in rabbits immunized with E. coli-derived Human FAM219B fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAM219B Antibody - Azide and BSA Free
Western Blot: FAM219B Antibody - Azide and BSA Free [NBP3-26092] -
Western Blot: FAM219B Antibody - Azide and BSA Free [NBP3-26092] - NBP3-26092 at 1:500 dilution Lane A: ACHN Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:26 kDaImmunocytochemistry/ Immunofluorescence: FAM219B Antibody - Azide and BSA Free [NBP3-26092] -
Immunocytochemistry/ Immunofluorescence: FAM219B Antibody - Azide and BSA Free [NBP3-26092] - Immunofluorescence staining of FAM219B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-26092 (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green). Positive staining was localized to Nucleus.Applications for FAM219B Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS, pH 7.0
Format
Azide and BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAM219B
Alternate Names
C15orf17, chromosome 15 open reading frame 17, FLJ00005, hypothetical protein LOC57184
Gene Symbol
FAM219B
Additional FAM219B Products
Product Documents for FAM219B Antibody - Azide and BSA Free
Product Specific Notices for FAM219B Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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