FANCD2 Antibody (1290D) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54808
Recombinant Monoclonal Antibody.
![Flow (Intracellular): FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Flow-Intracellular-NBP2-54808-img0005.jpg "Flow (Intracellular): FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]")
![Western Blot: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Western-Blot-NBP2-54808-img0006.jpg "Western Blot: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]")
![Knockout Validated: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Knockout-Validated-NBP2-54808-img0003.jpg "Knockout Validated: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1290D
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This FANCD2 Antibody (1290D) was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FANCD2 Antibody (1290D) - BSA Free
Knockout Validated: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]
Knockout Validated: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Simple Western: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Simple-Western-NBP2-54808-img0002.jpg "Simple Western: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]")
Simple Western: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]
Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate.![Immunohistochemistry: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]](https://resources.bio-techne.com/images/products/FANCD2-Antibody-1290D-Immunohistochemistry-NBP2-54808-img0004.jpg "Immunohistochemistry: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]")
Immunohistochemistry: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]
Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.Applications for FANCD2 Antibody (1290D) - BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunohistochemistry
5-10 ug/ml
Immunohistochemistry-Paraffin
5-10 ug/ml
Simple Western
1 ug/ml
Western Blot
0.1 - 0.5 ug/ml
Reviewed Applications
Read 1 review rated 4 using NBP2-54808 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FANCD2
References
1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014
2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1
Long Name
Fanconi Anemia, Complementation Group D2
Alternate Names
FA4, FACD, FAD, FAD2, FANCD
Gene Symbol
FANCD2
Additional FANCD2 Products
Product Documents for FANCD2 Antibody (1290D) - BSA Free
Product Specific Notices for FANCD2 Antibody (1290D) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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