FANCD2 [p Ser222] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-502

Novus Biologicals, part of Bio-Techne
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NB100-502
NB100-502SS
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all FANCD2 Antibodies »

Product Specifications

Immunogen

This FANCD2 [p Ser222] Antibody was developed against a synthetic peptide made to human FANCD2 (residues 217-226), surrounding the phosphorylated Serine 222. [Swiss-Prot# Q9BXW9]

Modification

p Ser222

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FANCD2 [p Ser222] Antibody

Western Blot: FANCD2 [p Ser222] Antibody [NB100-502]

Western Blot: FANCD2 [p Ser222] Antibody [NB100-502]

Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - Indicated lymphoblasts (PD7, WT: GM1526, AT) were irradiated with 15 Gy (2), and immunoblotted with anti-FANCD2 and anti-FANCD2 (pS-222)
Western Blot: FANCD2 [p Ser222] Antibody [NB100-502] - FA-D2 fibroblasts were stably transfected with either pMMP (empty vector, A), FANCD2 (wt, B), FANCD2 [p Ser222] Antibody (C), FANCD2 (triple mutant, D), or FANCD2 (quadruple mutant, E).

Applications for FANCD2 [p Ser222] Antibody

Application
Recommended Usage

Western Blot

1:100-1:500
Application Notes
This FANCD2 [p Ser222] antibody is useful for Western Blot. 1:400 dilution recommended for PD20 FANCD2 overexpressing cells, 1:100 for HeLa cells, 1:200 for other cell types.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FANCD2

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Long Name

Fanconi Anemia, Complementation Group D2

Alternate Names

FA4, FACD, FAD, FAD2, FANCD

Gene Symbol

FANCD2

Additional FANCD2 Products

Product Documents for FANCD2 [p Ser222] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FANCD2 [p Ser222] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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