FANCF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77986
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified FANCF Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal amino acid sequence of human FANCF. (Uniprot: Q9NPI8)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with FANCF protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against human FANCF protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for FANCF Antibody
![Western Blot: FANCF Antibody [NBP1-77986]](https://resources.bio-techne.com/images/products/FANCF-Antibody-Western-Blot-NBP1-77986-img0001.jpg "Western Blot: FANCF Antibody [NBP1-77986]")
Western Blot: FANCF Antibody [NBP1-77986]
Western Blot: FANCF Antibody [NBP1-77986] - anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific.Applications for FANCF Antibody
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kDa in size corresponding to FANCF by western blotting in the appropriate human tissue.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: FANCF
Alternate Names
FAF, Fanconi anemia group F protein, Fanconi anemia, complementation group F, MGC126856, Protein FACF
Gene Symbol
FANCF
UniProt
Additional FANCF Products
Product Documents for FANCF Antibody
Product Specific Notices for FANCF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...