FANCG Antibody (2C8)
Novus Biologicals, part of Bio-Techne | Catalog # H00002189-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2C8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
FANCG (AAH11623, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL
Specificity
FANCG - Fanconi anemia, complementation group G
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for FANCG Antibody (2C8)
Western Blot: FANCG Antibody (2C8) [H00002189-M01]
Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.Lane 1: FANCG transfected lysate(68.6 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01]
Immunocytochemistry/Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01] - Analysis of monoclonal antibody to FANCG on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: FANCG Antibody (2C8) [H00002189-M01]
Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG over-expressed 293 cell line, cotransfected with FANCG Validated Chimera RNAi ( Cat # H00002189-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FANCG monoclonal antibody (M01), clone 2C8 (Cat # H00002189-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for FANCG Antibody (2C8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunoprecipitation
1:10-1:1000
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: FANCG
Long Name
Fanconi Anemia Complementation Group G
Alternate Names
XRCC9
Entrez Gene IDs
2189 (Human)
Gene Symbol
FANCG
OMIM
602956 (Human)
UniProt
Additional FANCG Products
Product Documents for FANCG Antibody (2C8)
Product Specific Notices for FANCG Antibody (2C8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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