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FANCG Antibody (2C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00002189-M01

Novus Biologicals, part of Bio-Techne
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H00002189-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

FANCG (AAH11623, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL

Specificity

FANCG - Fanconi anemia, complementation group G

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FANCG Antibody (2C8)

Western Blot: FANCG Antibody (2C8) [H00002189-M01]

Western Blot: FANCG Antibody (2C8) [H00002189-M01]

Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.Lane 1: FANCG transfected lysate(68.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01]

Immunocytochemistry/ Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01]

Immunocytochemistry/Immunofluorescence: FANCG Antibody (2C8) [H00002189-M01] - Analysis of monoclonal antibody to FANCG on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: FANCG Antibody (2C8) [H00002189-M01]

Western Blot: FANCG Antibody (2C8) [H00002189-M01]

Western Blot: FANCG Antibody (2C8) [H00002189-M01] - Analysis of FANCG over-expressed 293 cell line, cotransfected with FANCG Validated Chimera RNAi ( Cat # H00002189-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FANCG monoclonal antibody (M01), clone 2C8 (Cat # H00002189-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for FANCG Antibody (2C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:1000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FANCG

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G.

Long Name

Fanconi Anemia Complementation Group G

Alternate Names

XRCC9

Entrez Gene IDs

2189 (Human)

Gene Symbol

FANCG

OMIM

602956 (Human)

Additional FANCG Products

Product Documents for FANCG Antibody (2C8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FANCG Antibody (2C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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