Fascin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91887
![Western Blot: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Western-Blot-NBP1-91887-img0014.jpg "Western Blot: Fascin Antibody [NBP1-91887]")
![Immunocytochemistry/ Immunofluorescence: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91887-img0010.jpg "Immunocytochemistry/ Immunofluorescence: Fascin Antibody [NBP1-91887]")
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Fascin Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ
Marker
Dendritic Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Fascin Antibody
![Knockdown Validated: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Knockdown-Validated-NBP1-91887-img0021.jpg "Knockdown Validated: Fascin Antibody [NBP1-91887]")
Knockdown Validated: Fascin Antibody [NBP1-91887]
Knockdown Validated: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0018.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]")
Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]
Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP1-91887 (A) shows similar protein distribution across tissues to independent antibody NBP2-58042 (B).![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunohistochemistry-NBP1-91887-img0020.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]")
![Western Blot: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Western-Blot-NBP1-91887-img0008.jpg "Western Blot: Fascin Antibody [NBP1-91887]")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0015.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0017.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]](https://resources.bio-techne.com/images/products/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0019.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887]")
Applications for Fascin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fascin
Alternate Names
FAN1, FSCN1, HSN, Singed-like Protein, SNL
Gene Symbol
FSCN1
Additional Fascin Products
Product Documents for Fascin Antibody
Product Specific Notices for Fascin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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