Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FAT1 Antibodies »

Product Summary for FAT1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 4450 to 4500 of human FAT Tumor Suppressor Homolog 1 using the numbering given in entry NP_005236.2 (GeneID 2195).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT1 Antibody

Immunoprecipitation: FAT1 Antibody [NBP2-32274]

Immunoprecipitation: FAT1 Antibody [NBP2-32274]

Immunoprecipitation: FAT1 Antibody [NBP2-32274] - Detection of human FAT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAT1 antibody NBP2-32274 (lot NBP2-32274-2) used for IP at 6 ug per reaction. FAT1 was also immunoprecipitated by a previous lot of this antibody (lot NBP2-32274-1) and rabbit anti-FAT1 antibody NBP2-32275. For blotting immunoprecipitated FAT1, NBP2-32274 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
FAT1 Antibody

Western Blot: FAT1 Antibody [NBP2-32274] -

Western Blot: FAT1 Antibody [NBP2-32274] - : Wholecell lysate (2 ug) from Hep-G2, RKO, HeLa, Jurkat, andHEK293T cells prepared using NETN lysis buffer.Antibodies: Affinity purified rabbit anti-FAT1 antibodyused for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.
FAT1 Antibody

Western Blot: FAT1 Antibody [NBP2-32274] -

Western Blot: FAT1 Antibody [NBP2-32274] - Wholecell lysate (2 ug) from NIH 3T3, CT26, CH27, TCMK-1, andBW5147.3 cells prepared using NETN lysis buffer.Antibodies: Affinity purified rabbit anti-FAT1 antibody used for WB at 0.04 ug/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

Applications for FAT1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FAT1

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Alternate Names

FAT tumor suppressor 1, FAT tumor suppressor homolog 1 (Drosophila)

Gene Symbol

FAT1

UniProt

NP_005236.2 (Human)

Additional FAT1 Products

Product Documents for FAT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FAT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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