Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FAT10 Antibodies »

Product Specifications

Immunogen

FAT10 antibody was raised against a 16 amino acid peptide near the center of human FAT10. The immunogen is located within amino acids 80 - 130 of FAT10. Amino Acid Squence: ESGDEAKRHLLQVRRS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat: (81%)

Specificity

FAT10 antibody is human specific.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FAT10 Antibody - BSA Free

Western Blot: FAT10 AntibodyBSA Free [NBP2-81752]

Western Blot: FAT10 AntibodyBSA Free [NBP2-81752]

Western Blot: FAT10 Antibody [NBP2-81752] - Analysis of FAT10 in human testis tissue lysate with FAT10 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: FAT10 Antibody - BSA Free [NBP2-81752]

Immunocytochemistry/ Immunofluorescence: FAT10 Antibody - BSA Free [NBP2-81752]

Immunocytochemistry/Immunofluorescence: FAT10 Antibody [NBP2-81752] - Immunofluorescence of FAT10 in human testis tissue with FAT10 antibody at 20 ug/ml.
Immunohistochemistry: FAT10 Antibody - BSA Free [NBP2-81752]

Immunohistochemistry: FAT10 Antibody - BSA Free [NBP2-81752]

Immunohistochemistry: FAT10 Antibody [NBP2-81752] - Immunohistochemistry of FAT10 in human testis tissue with FAT10 antibody at 5 ug/mL.
View 4 more images

Applications for FAT10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAT10

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10. Human FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, but more than one mRNA transcript has been identified for murine FAT10. FAT10 can also be induced by IFN-gamma and TNF-alpha in some cell lines. Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10-conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein. In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L. FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response.

Alternate Names

UBD

Gene Symbol

UBD

Additional FAT10 Products

Product Documents for FAT10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FAT10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.