FAT4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78381
![Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]](https://resources.bio-techne.com/images/products/FAT4-Antibody-Knockout-Validated-NBP1-78381-img0006.jpg "Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381]")
![Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381]](https://resources.bio-techne.com/images/products/FAT4-Antibody-Immunohistochemistry-NBP1-78381-img0004.jpg "Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381]")
![Knockout Validated: FAT4 Antibody - BSA Free [NBP1-78381]](https://resources.bio-techne.com/images/products/FAT4-Antibody-Knockout-Validated-NBP1-78381-img0005.jpg "Immunohistochemistry: FAT4 Antibody - BSA Free [NBP1-78381]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7]
Localization
Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAT4 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: FAT4 Antibody - BSA Free [NBP1-78381]](https://resources.bio-techne.com/images/products/FAT4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78381-img0003.jpg "Immunocytochemistry/ Immunofluorescence: FAT4 Antibody - BSA Free [NBP1-78381]")
Immunocytochemistry/ Immunofluorescence: FAT4 Antibody - BSA Free [NBP1-78381]
Immunocytochemistry/Immunofluorescence: FAT4 Antibody [NBP1-78381] - Antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with DAPI (blue).Applications for FAT4 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
reported in scientific literature (PMID 29321622)
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAT4
Alternate Names
cadherin family member 14, cadherin-related family member 11, CDHR11, FAT tumor suppressor homolog 4 (Drosophila), FAT-J, fat-like cadherin protein FAT-J, FLJ77878, hFat4, NBLA00548, putative protein product of Nbla00548
Entrez Gene IDs
79633 (Human)
Gene Symbol
FAT4
UniProt
Additional FAT4 Products
Product Documents for FAT4 Antibody - BSA Free
Product Specific Notices for FAT4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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