Fatty Acid Synthase/FASN Antibody (4U9S3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15634

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15634-100ul
NBP3-15634-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4U9S3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fatty Acid Synthase/FASN Antibodies »

Product Summary for Fatty Acid Synthase/FASN Antibody (4U9S3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human FASN (P49327). AVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fatty Acid Synthase/FASN Antibody (4U9S3)

Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Western Blot: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of extracts of various cell lines, using Fatty Acid Synthase/FASN antibody (NBP3-15634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Analysis of NIH-3T3 cells using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody (4U9S3) [NBP3-15634] - Human colon carcinoma using Fatty Acid Synthase/FASN Rabbit mAb (NBP3-15634) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Fatty Acid Synthase/FASN Antibody (4U9S3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Alternate Names

FASN, OA-519, SDR27X1

Gene Symbol

FASN

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody (4U9S3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fatty Acid Synthase/FASN Antibody (4U9S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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