FBX09 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27364

Novus Biologicals, part of Bio-Techne
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NBP2-27364
NBP2-27364SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all FBX09 Antibodies »

Product Summary for FBX09 Antibody

Immunogen

Synthetic peptide made to an N-terminal sequence of the human FBX09 protein (between amino acids 1-100) [UniProt Q9UK97]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous to most ape species, most monkey species (95%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBX09 Antibody

Western Blot: FBX09 Antibody [NBP2-27364]

Western Blot: FBX09 Antibody [NBP2-27364]

Western Blot: FBX09 Antibody [NBP2-27364] - Analysis of FBXO9 using FBXO9 polyclonal antibody. ThP1 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with FBXO9 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: FBX09 Antibody [NBP2-27364] - ICC-IF validation of FBX09 antibody on HeLa cells at 1:100 dilution with detection using Dylight 488 - conjugated secondary antibody (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: FBX09 Antibody [NBP2-27364]

Immunohistochemistry-Paraffin: FBX09 Antibody [NBP2-27364]

Immunohistochemistry-Paraffin: FBX09 Antibody [NBP2-27364] - IHC analysis of formalin fixed paraffin embedded tissue section of human pancreas using FBX09 antibody at 1:100 dilution. The antibody generated a cytoplasmic-nuclear staining in cells of Islets of Langerhans while the pancreatic acini showed very mild to negligible signal for FBX09 immunostaining.

Applications for FBX09 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5ug/ml

Immunohistochemistry

2-5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Western Blot

4-6ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS containing 0.05% BSA.

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBX09

FBXO9 (F-box protein 9) is a Ubl conjugation pathway protein and it contains one F-box domain and one TPR repeat. FBXO9 may act as a potential sepsis biomarker or therapeutic target. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SkP1-cullin-F-box), which functions in phosphorylation-dependent ubiquitination.

Alternate Names

Cross-immune reaction antigen 1, dJ341E18.2, F-box only protein 9, F-box protein 9, F-box protein Fbx9, FBX9DKFZp434C0118, KIAA0936, NY-REN-57, Renal carcinoma antigen NY-REN-57, VCIA1

Gene Symbol

FBXO9

UniProt

Q9UK97 (Human)

Additional FBX09 Products

Product Documents for FBX09 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FBX09 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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