Fbx32 Antibody (1F10Z5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16255
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1F10Z5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Fbx32 Antibody (1F10Z5)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Fbx32 (Q969P5). MPFLGQDWRSPGQNWVKTADGWKRFLDEKSGSFVSDLSSYCNKEVYNKENLFNSLNYDVAAKKRKKDMLNSKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Fbx32 Antibody (1F10Z5)
![Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255]](https://resources.bio-techne.com/images/products/Fbx32-Antibody-1F10Z5-Western-Blot-NBP3-16255-img0002.jpg "Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255]")
Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255]
Western Blot: Fbx32 Antibody (1F10Z5) [NBP3-16255] - Western blot analysis of extracts of various cell lines, using Fbx32 Rabbit mAb (NBP3-16255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255]](https://resources.bio-techne.com/images/products/Fbx32-Antibody-1F10Z5-Immunohistochemistry-Paraffin-NBP3-16255-img0001.jpg "Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255]")
Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255]
Immunohistochemistry-Paraffin: Fbx32 Antibody (1F10Z5) [NBP3-16255] - Rat heart using Fbx32 Rabbit mAb (NBP3-16255) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Fbx32 Antibody (1F10Z5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Fbx32
Alternate Names
atrogin 1, ATROGIN1, atrogin-1, F-box only protein 32, F-box protein 32, Fbx32, FLJ32424, MAFBX, MAFbxMGC33610, Muscle atrophy F-box protein
Gene Symbol
FBXO32
Additional Fbx32 Products
Product Documents for Fbx32 Antibody (1F10Z5)
Product Specific Notices for Fbx32 Antibody (1F10Z5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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