Fbx32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36955

Novus Biologicals, part of Bio-Techne
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NBP1-36955
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Fbx32 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NSKTKTQYFHQEK corresponding to internal region according to NP_478136.1.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28068319).

Specificity

This antibody is expected to recognize reported isoform 1 (NP_478136.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fbx32 Antibody

Western Blot: Fbx32 Antibody [NBP1-36955]

Western Blot: Fbx32 Antibody [NBP1-36955]

Western Blot: Fbx32 Antibody [NBP1-36955] - Staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Fbx32 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/mL
Application Notes
WB: Approx. 40 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 41.6 kDa band according to human NP_478136.1 and of 41.5 kDa band according to mouse NP_080622.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fbx32

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing of this gene results in two transcript variants encoding two isoforms of different sizes. [provided by RefSeq]

Alternate Names

atrogin 1, ATROGIN1, atrogin-1, F-box only protein 32, F-box protein 32, Fbx32, FLJ32424, MAFBX, MAFbxMGC33610, Muscle atrophy F-box protein

Entrez Gene IDs

114907 (Human); 67731 (Mouse); 171043 (Rat)

Gene Symbol

FBXO32

UniProt

Q969P5 (Human)

Additional Fbx32 Products

Product Documents for Fbx32 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fbx32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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