Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all FBXL20 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER. The immunogen is located within the first 50 amino acids of SCRAPPER. Amino Acid Squence: SDEAVINKKLPKELLLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXL20 Antibody - BSA Free

Western Blot: FBXL20 AntibodyBSA Free [NBP1-77317]

Western Blot: FBXL20 AntibodyBSA Free [NBP1-77317]

Western Blot: FBXL20 Antibody [NBP1-77317] - A20 cell lysate with FBXL20 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunohistochemistry-Paraffin: FBXL20 Antibody - BSA Free [NBP1-77317]

Immunohistochemistry-Paraffin: FBXL20 Antibody - BSA Free [NBP1-77317]

Immunohistochemistry-Paraffin: FBXL20 Antibody [NBP1-77317] - Human brain tissue using FBXL20 antibody at 2.5 ug/ml.

Immunohistochemistry: FBXL20 Antibody [NBP1-77317] -

Immunohistochemistry: FBXL20 Antibody [NBP1-77317] - Immunofluorescence of scrapper in human brain tissue with scrapper antibody at 20 ug/mL.
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Applications for FBXL20 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXL20

Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

Alternate Names

DKFZp564O2364, Fbl2, Fbl20, F-box and leucine-rich repeat protein 20FLJ21037, F-box protein FBL2, F-box/LRR-repeat protein 20, F-box/LRR-repeat protein 2-like, MGC15482

Gene Symbol

FBXL20

UniProt

NP_116264 (Human)

Additional FBXL20 Products

Product Documents for FBXL20 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FBXL20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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