FBXO11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-59826
![Western Blot: FBXO11 Antibody [NB100-59826]](https://resources.bio-techne.com/images/products/FBXO11-Antibody-Western-Blot-NB100-59826-img0004.jpg "Western Blot: FBXO11 Antibody [NB100-59826]")
![Western Blot: FBXO11 Antibody [NB100-59826]](https://resources.bio-techne.com/images/products/FBXO11-Antibody-Western-Blot-NB100-59826-img0006.jpg "Western Blot: FBXO11 Antibody [NB100-59826]")
![Western Blot: FBXO11 Antibody [NB100-59826]](https://resources.bio-techne.com/images/products/FBXO11-Antibody-Western-Blot-NB100-59826-img0002.jpg "Western Blot: FBXO11 Antibody [NB100-59826]")
![Simple Western: FBXO11 Antibody [NB100-59826]](https://resources.bio-techne.com/images/products/FBXO11-Antibody-Simple-Western-NB100-59826-img0003.jpg "Simple Western: FBXO11 Antibody [NB100-59826]")
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 877 and 927 of human F-box only protein 11 using the numbering given in entry NP_079409.3 (GeneID 80204).
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FBXO11 Antibody
![Knockout Validated: FBXO11 Antibody [NB100-59826]](https://resources.bio-techne.com/images/products/FBXO11-Antibody-Knockout-Validated-NB100-59826-img0005.jpg "Knockout Validated: FBXO11 Antibody [NB100-59826]")
Knockout Validated: FBXO11 Antibody [NB100-59826]
Knockout Validated: FBXO11 Antibody [NB100-59826] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FBXO11 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human FBXO11 Polyclonal Antibody (Catalog # NB100-59826) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FBXO11 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Applications for FBXO11 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Simple Western
1:40
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:34505148). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. FBXO11antibody validated for WB from verified customer reviews.
Reviewed Applications
Read 4 reviews rated 4.5 using NB100-59826 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FBXO11
Alternate Names
F-box only protein 11, F-box protein 11, FBX11MGC44383, FLJ12673, PRMT9VIT1protein arginine N-methyltransferase 9, ubiquitin protein ligase E3 component n-recognin 6, UBR6, VIT-1, Vitiligo-associated protein 1, vitiligo-associated protein VIT-1
Entrez Gene IDs
80204 (Human)
Gene Symbol
FBXO11
UniProt
Additional FBXO11 Products
Product Documents for FBXO11 Antibody
Product Specific Notices for FBXO11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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