Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FBXO31 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 489 and 539 of human F-box protein 31 using the numbering given in entry NP_079011.3 (GeneID 79791).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO31 Antibody

Western Blot: FBXO31 Antibody [NBP1-19088]

Western Blot: FBXO31 Antibody [NBP1-19088]

Western Blot: FBXO31 Antibody [NBP1-19088] - Detection of Human and Mouse FBXO31 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FBXO31 antibody NBP1-19088 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for FBXO31 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID 24623383)

Immunoprecipitation

Negative

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FBXO31

Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Alternate Names

DKFZp434B027, F-box only protein 31, F-box protein 31, FBX14DKFZp434J1815, Fbx31, FBXO14FLJ22477, MGC15419, MGC9527, pp2386, putative breast cancer tumor-suppressor, SCF ubiquitin ligase specificity factor

Entrez Gene IDs

79791 (Human); 76454 (Mouse)

Gene Symbol

FBXO31

UniProt

Q5XUX0 (Human)

Additional FBXO31 Products

Product Documents for FBXO31 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FBXO31 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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