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FBXO4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16450

Novus Biologicals, part of Bio-Techne
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NBP2-16450

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for FBXO4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXO4. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO4 Antibody

Western Blot: FBXO4 Antibody [NBP2-16450]

Western Blot: FBXO4 Antibody [NBP2-16450]

Western Blot: FBXO4 Antibody [NBP2-16450] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: FBXO4 Antibody [NBP2-16450]

Immunocytochemistry/ Immunofluorescence: FBXO4 Antibody [NBP2-16450]

Immunocytochemistry/Immunofluorescence: FBXO4 Antibody [NBP2-16450] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using FBXO4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Applications for FBXO4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FBXO4

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Alternate Names

DKFZp547N213, F-box protein 4, F-box protein Fbx4, FBX4F-box only protein 4, FLJ10141

Gene Symbol

FBXO4

UniProt

Additional FBXO4 Products

Product Documents for FBXO4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXO4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.