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FBXW7/Cdc4 Antibody (PD00-51)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32335

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32335

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PD00-51

Concentration

1 mg/ml

Product Summary for FBXW7/Cdc4 Antibody (PD00-51)

Immunogen

Synthetic peptide. (Uniprot: Q969H0)

Localization

Chromosome, Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXW7/Cdc4 Antibody (PD00-51)

FBXW7/Cdc4 Antibody (PD00-51)

Immunohistochemistry: FBXW7/Cdc4 Antibody (PD00-51) [NBP3-32335]

Immunohistochemistry: FBXW7/Cdc4 Antibody (PD00-51) [NBP3-32335] - Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-FBXW7/Cdc4 antibody (NBP3-32335) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32335) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for FBXW7/Cdc4 Antibody (PD00-51)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXW7/Cdc4

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Long Name

F-box and WD Repeat Domain Containing 7

Alternate Names

AGO, Archipelago Homolog, Cdc4, FBW6, Fbw7, FBX30, FBXO30, FBXW6, SCF(Fbw7), SEL10

Gene Symbol

FBXW7

Additional FBXW7/Cdc4 Products

Product Documents for FBXW7/Cdc4 Antibody (PD00-51)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXW7/Cdc4 Antibody (PD00-51)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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