Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FBXW7/Cdc4 Antibodies »

Product Summary for FBXW7/Cdc4 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human F-box and WD-40 domain protein 7 using the numbering given in entry NP_361014.1 (GeneID 55294).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FBXW7/Cdc4 Antibody

Immunohistochemistry: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry: FBXW7/Cdc4 Antibody [NB100-88139] - Detection of human FBW7 by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FBW7 (NB100-88139). Detection: DAB.
Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-FBW7 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139]

Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
View 2 more images

Applications for FBXW7/Cdc4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500

Immunoprecipitation

2-5 mcg/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FBXW7/Cdc4

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Long Name

F-box and WD Repeat Domain Containing 7

Alternate Names

AGO, Archipelago Homolog, Cdc4, FBW6, Fbw7, FBX30, FBXO30, FBXW6, SCF(Fbw7), SEL10

Entrez Gene IDs

55294 (Human)

Gene Symbol

FBXW7

UniProt

Q969H0 (Human)

Additional FBXW7/Cdc4 Products

Product Documents for FBXW7/Cdc4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FBXW7/Cdc4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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