Fc epsilon RI Antibody (43160) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43279

Novus Biologicals, part of Bio-Techne
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NBP1-43279-0.1mg
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 43160

Format

BSA Free

Concentration

0.5 mg/ml

View all Fc epsilon RI Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was FceRI alpha.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for Fc epsilon RI Antibody (43160) - BSA Free

Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the FITC conjugate of NBP1-43279. Staining of the MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (filled histogram). Total
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]

Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the Biotin conjugate of NBP1-43279. Staining of MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) Biotin (filled histogram)followed by Streptavidin PE . Total viable cells were use

Applications for Fc epsilon RI Antibody (43160) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Fc epsilon RI

The MAR-1 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Alternate Names

alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide

Gene Symbol

FCER1A

Additional Fc epsilon RI Products

Product Documents for Fc epsilon RI Antibody (43160) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fc epsilon RI Antibody (43160) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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