Fc epsilon RI Antibody (43160) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43279
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # 43160
Format
BSA Free
Concentration
0.5 mg/ml
Product Summary for Fc epsilon RI Antibody (43160) - BSA Free
Immunogen
The immunogen for this antibody was FceRI alpha.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for Fc epsilon RI Antibody (43160) - BSA Free
Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the FITC conjugate of NBP1-43279. Staining of the MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control FITC (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (filled histogram). TotalFlow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Surface staining of MC/9 cell line with Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.Flow Cytometry: Fc epsilon RI Antibody (43160) - BSA Free [NBP1-43279]
Flow Cytometry: Fc epsilon RI Antibody (43160) [NBP1-43279] - Analysis using the Biotin conjugate of NBP1-43279. Staining of MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) Biotin (filled histogram)followed by Streptavidin PE . Total viable cells were useApplications for Fc epsilon RI Antibody (43160) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Fc epsilon RI
Alternate Names
alpha polypeptide, Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, Fc-epsilon RI-alpha, FcERI, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, high-affinity, of mast cells, alpha polypeptide
Gene Symbol
FCER1A
Additional Fc epsilon RI Products
Product Documents for Fc epsilon RI Antibody (43160) - BSA Free
Product Specific Notices for Fc epsilon RI Antibody (43160) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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