Fc gamma RI/CD64 Antibody (027)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90405
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]](https://resources.bio-techne.com/images/products/Fc-gamma-RI-CD64-Antibody-027-Immunocytochemistry-Immunofluorescence-NBP2-90405-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 027
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Fc gamma RI/CD64 Antibody (027)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Fc gamma RI/CD64 (Accession#: NP_034316.1; Met1-Pro297).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Fc gamma RI/CD64 Antibody (027)
Immunocytochemistry/ Immunofluorescence: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]
Immunocytochemistry/Immunofluorescence: Fc gamma RI/CD64 Antibody (027) [NBP2-90405] - Immunofluorescence staining of mouse Fc gamma RI/CD64 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse Fc gamma RI/CD64 monoclonal antibody (1:60) at 37C 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.![Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]](https://resources.bio-techne.com/images/products/Fc-gamma-RI-CD64-Antibody-027-Flow-Cytometry-NBP2-90405-img0001.jpg "Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]")
Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405]
Flow Cytometry: Fc gamma RI/CD64 Antibody (027) [NBP2-90405] - Flow cytometric analysis of Mouse Fc gamma RI/CD64 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse Fc gamma RI/CD64, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for Fc gamma RI/CD64 Antibody (027)
Application
Recommended Usage
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fc gamma RI/CD64
Long Name
Fc gamma Receptor I
Alternate Names
CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA
Gene Symbol
FCGR1A
Additional Fc gamma RI/CD64 Products
Product Documents for Fc gamma RI/CD64 Antibody (027)
Product Specific Notices for Fc gamma RI/CD64 Antibody (027)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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