Fc gamma RII/CD32 Antibody (AT10)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65338
![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]](https://resources.bio-techne.com/images/products/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0005.jpg "Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Porcine
Applications
Validated:
Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # AT10
Concentration
1.0 mg/ml
Product Specifications
Immunogen
K562 cell line
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID:33114132).
Specificity
NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Fc gamma RII/CD32 Antibody (AT10)
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.![Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]](https://resources.bio-techne.com/images/products/Fc-gamma-RII-CD32-Antibody-AT10-Flow-Cytometry-NB100-65338-img0004.jpg "Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]")
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.Applications for Fc gamma RII/CD32 Antibody (AT10)
Application
Recommended Usage
Flow Cytometry
20 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:500-1:1000
Immunoprecipitation
20 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using NB100-65338 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fc gamma RII/CD32
Long Name
Fc gamma Receptor II
Alternate Names
FcgRII
Gene Symbol
FCGR2B
Additional Fc gamma RII/CD32 Products
Product Documents for Fc gamma RII/CD32 Antibody (AT10)
Product Specific Notices for Fc gamma RII/CD32 Antibody (AT10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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