Fc gamma RII/CD32 Antibody (FCGR2A/479) [Janelia Fluor® 585]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08220JF585
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Janelia Fluor 585
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # FCGR2A/479
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human Fc gamma RII/CD32 protein (Uniprot: P12318)
Localization
Cell Surface
Specificity
This monoclonal antibody binds most strongly to the IIa isoforms of CD32 (FcRII), a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Applications for Fc gamma RII/CD32 Antibody (FCGR2A/479) [Janelia Fluor® 585]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fc gamma RII/CD32
Long Name
Fc gamma Receptor II
Alternate Names
FcgRII
Gene Symbol
FCGR2B
Additional Fc gamma RII/CD32 Products
Product Documents for Fc gamma RII/CD32 Antibody (FCGR2A/479) [Janelia Fluor® 585]
Product Specific Notices for Fc gamma RII/CD32 Antibody (FCGR2A/479) [Janelia Fluor® 585]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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