Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70709

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Fc gamma RIIA/CD32a Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free

Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5)Azide and BSA Free [NBP2-70709]

Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5)Azide and BSA Free [NBP2-70709]

Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]

Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]

Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]

Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]

Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.
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Applications for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:200 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fc gamma RIIA/CD32a

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Gene Symbol

FCGR2A

Additional Fc gamma RIIA/CD32a Products

Product Documents for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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